The Powerpuff Girls — DC Comics
Issue: #14
D-uh, it's the Amoeba Boys' first appearance in THE POWERPUFF GIRLS, boss! When Townsville's lamest villains decide to become Townsville's lousiest superheroes, the Girls rush to stop the Boys in their slimy tracks! Yuck!
First appearances: Junior, Bossman, Slim
Creators: Harvey Richards, Dave Tanguay, Ryan Cline, Joan Hilty, John Rozum, Philip Moy
Characters: Bubbles, Blossom, Mojo Jojo, Ms. Bellum, Junior, Bossman, Buttercup, Slim, Mayor